Post Doctoral Research Associate
University of Cambridge
Cambridge CB2 1LR
My primary research interest focusses on the mechanisms microbes adopt to integrate multiple stimuli and give out a coordinated response. I am interested in understanding the gene circuitry responsible for this mechanism. Transcription of effector genes is a major regulatory step in any response pathway, and recent developments in single cell studies have thrown light on the transcriptional level heterogeneity for many genes even in isogenic populations. I have chosen to work on signal integration at the single cell level leading to transcription regulation. I will be using cyanobacteria as a model organism to gain quantitative insights into gene regulation circuits and how their dynamic behaviour at single cell level alters the overall transcription landscape and affects the survival of the organism.
Unique N-terminal Arm of Mycobacterium tuberculosis PhoP Protein Plays an Unusual Role in Its Regulatory Function. Das AK, Kumar VA, Sevalkar RR, Bansal R, Sarkar D. J Biol Chem. 2013 Oct 4;288(40):29182-92
Phosphorylation of PhoP protein plays direct regulatory role in lipid biosynthesis of Mycobacterium tuberculosis. Goyal R, Das AK, Singh R, Singh PK, Korpole S, Sarkar D. J Biol Chem. 2011 Dec 30;286(52):45197-208
A single-amino-acid substitution in the C terminus of PhoP determines DNA-binding specificity of the virulence-associated response regulator from Mycobacterium tuberculosis. Das AK, Pathak A, Sinha A, Datt M, Singh B, Karthikeyan S, Sarkar D. J Mol Biol. 2010 May 21;398(5):647-56.