Researchers have produced the most detailed map yet of how stem cells in the growing tip of a plant begin their journey to form the many cell types that shape flowers and stems.
They identified 18 distinct cell type clusters in the inflorescence meristem (the stem cell niche at the tip of the flowering shoot) that generates a plant’s above-ground organs such as stems and flowers.
The cell groups identified include precursors to the plant cortex (a tissue layer that helps support the plant and store nutrients) and vascular cell tissues, including phloem, xylem and cambium. This indicates that tissue patterning starts much earlier in the meristem than previously thought, with distinct groups of cells diverging during primordia formation.
Inflorescence meristem cell identities: (A) Force-directed graph layout of clusters associated with inner cell layers such as early primordia (EP), undifferentiated cells, procambium, xylem parenchyma and cortex. Clusters are represented by different colours. (B) Schematic illustration of selected cell identities in the primary stem and inflorescence meristem. Procambial strands arise in developing primordia and give rise to vascular bundles, which subsequently differentiate into phloem, cambium and xylem within the primary stem. The exact locations of cell identities in the primary stem are schematically represented rather than accurately portrayed. Illustration by first author first author Dr Sebastián Moreno-Ramírez. Source: Fig 6 in Moreno-Ramirez et al Science Advances 2026.
Published in Science Advances, the research, led through a collaboration between the research groups of Henrik Jönsson, Elliot Meyerowitz and James Locke at the Sainsbury Laboratory Cambridge University (SLCU), provides new insights into how the inflorescence meristem generates the remarkable diversity of cell types that make up a plant’s above-ground organs.
Unlike animals, plants continue producing new organs throughout their lives. This lifelong growth depends on populations of stem cells being maintained within specialised tissues called meristems.
The inflorescence meristem continuously produces cells that will develop into flowers, stems and the vascular tissues that transport water and nutrients around the plant.
Although scientists already know the meristem contains distinct functional regions, it is not clear how stem cells make the transition from an undifferentiated state to specialised cell types.
This is particularly fascinating as cells sitting right next to each other may end up with totally different fates – one cell becomes the water conducting xylem while its neighbour becomes a petal.
Investigating the distinct cell types in the inflorescence meristem: Although we know some regulatory factors are important in certain domains in the inflorescence meristem, such as the zones where cytokinin (CT) and auxin response has been indentified (B), there are still many aspects that remain unknown in this stem cell niche. (A) The inflorescence meristem is responsible for the production of flowers and stems. The central zone (CZ) and organising centre (OC) are the domains dedicated to self-renewal and maintenance of pluripotency. The cell fates and regulatory factors at play in the rib zone (RZ), peripheral zone (PZ) are still relatively unknown. (C) This research paper has investigated the meristem in finer detail to reveal new developmental pathways and cell fates. Graphics and imaging by Sebastián Moreno-Ramírez.
How do cells determine their fates?
Using a relatively new technique called single-nucleus RNA-sequencing (snRNA-seq), the researchers traced these developmental pathways in unprecedented detail in the model plant Arabidopsis thaliana.
The study measured the expression of 19,491 genes in 10,025 individual nuclei extracted from finely dissected meristematic tissue.
By analysing the activity of genes (gene expression) in thousands of individual nuclei isolated from inflorescence meristems, lead author Dr Sebastián Moreno-Ramírez linked stems cells to their earliest differentiation states.
Using snRNA-seq: Schema with general workflow used for single-cell transcriptome analysis from dissected Arabidopsis thaliana inflorescence meristems. Floral meristems with distinct sepals were removed (> stage 4). Dissected meristems were frozen and then mechanically disrupted by pestle homogenization. Nuclei were FACS sorted before loading onto 10X Chromium chips. Scalebar = 100 µm. Source: Fig 1 in Moreno-Ramirez et al Science Advances 2026.
Single-nucleus RNA-sequencing (snRNA-seq)
Single-nucleus RNA sequencing (snRNA-seq) is emerging as a powerful tool for studying plant development because nuclei can be isolated from frozen tissues.
This is particularly valuable for small and hard-to-isolate tissues, such as the inflorescence meristem, because carefully dissected samples can be frozen and pooled over time. This allows researchers to enrich the dataset for cells from specific tissue of interest.
Instead of analysing the whole cell like traditional single-cell RNA sequencing, snRNA-seq isolates and sequences genetic materials from only the cell’s nucleus. The nuclei are released from tissue while preserving nuclear RNA, then isolated and sequenced.
This approach also reduces the risk that gene expression is affected during sample preparation.
snRNA-seq resolves cellular heterogeneity within the Arabidopsis thaliana infloresence meristem: Uniform manifold approximation (UMAP) and projection of cellular heterogeneity in the inflorescence meristem tissue (7,295 cell meristematic nuclei). This scatter plot uses nonlinear machine learning algorithm that compresses massive amounts of high-dimensional genomic data into a two-dimensional plot. It is used in plant biology to analyse cellular heterogeneity (the distinct genetic and functional variations between individual cells within plant tissues). Source: Fig 1 in Moreno-Ramirez et al Science Advances 2026.
Selected marker genes for each cell cluster in infloresence meristem: Source: Fig 6 in Moreno-Ramirez et al Science Advances 2026.
Using marker genes that they know are already more active in specific locations, the researchers were able to infer where different cell states arise in the meristem.
“We wanted to understand how a relatively small population of stem cells can continually generate the many different cell types needed to build a plant,” said Dr Moreno-Ramírez.
“By looking at gene expression of nuclei, we were able to infer developmental trajectories through big data analysis tools and advanced trajectory software to reconstruct developmental pathways.”
These trajectories revealed gene expression programmes associated with the formation of early floral organs and vascular cell types, including cambium, xylem and phloem.
Cortex and vasculature identities diverge during primordium formation within the inflorescence meristem: (left) Orthogonal and maximum projection of IM from pJKD::JKD-eYFP reporter line stained with PI. (right) Orthogonal slice of light-sheet image of pJKD::JKD-eYFP reporter line. Source: Fig 4 in Moreno-Ramirez et al Science Advances 2026.
The analysis also captured dynamic changes associated with progression through the cell cycle.
“Our data allowed us to follow the earliest steps of differentiation as stem cells commit to different developmental fates,” said Dr Moreno-Ramírez.
“Understanding these transitions is essential if we want to uncover the regulatory networks that control plant architecture and organ formation.”
By analysing genes expressed in specific domains, the researchers identified roles for members of the GH3 gene family, which participate in auxin metabolism. They linked these genes to meristem activity and the position of new organs, known as phyllotaxis.
Using GH3 mutant plants, the researchers observed altered growth patterns, confirming predictions generated from the snRNA-seq data set.
This data provides a valuable resource for future studies investigating how stem cells generate the diverse tissues that shape plant form.
“This gives us a framework for identifying the genetic networks that drive cell fate decisions in the meristem,” said Dr Moreno-Ramírez. “The next challenge is to understand how these regulatory programmes interact to control growth and to ensure that plants continuously produce organs and their cell types in the right place at the right time.”
Reference
Sebastián Moreno-Ramírez, Martin O. Lenz, Elliot M. Meyerowitz, James C.W. Locke, Henrik Jönsson (2026) Single-nucleus transcriptomics resolves multiple fate dynamics between inflorescence meristem and primary stem. Science Advances