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Leica-Renishaw SP8-FLIMan

A partnership between SLCU and three companies; Leica Microsystems, Leica Industrial and Renishaw, the FLIMan allows a diverse range of specialist techniques in addition to high-end confocal microscopy:

- Real time imaging of protein-protein interactions and biosensors using FLIM.

- Label-free organelle imaging using Raman, in addition to fluorescently labelled reporters.

- Direct imaging of molecular constituents of cells and tissues with applications to phytohormones.

Equipment

Leica SP8 confocal with dual channel FLIM, 2x HyD SMD detectors, 2x PMTs, 1x t-PMT, automatic stage for use in both confocal and Raman modes. Renishaw InVia Raman system with Rencam CCD and Andor EMCCD detectors. Conversion kit for liquid samples.

Software

Leica LAS X with FLIM module, Picoquant software for photon counting. WiRE software for Raman spectral acquisition and
mapping.
 

Lasers:

(Confocal) Pulsed 405nm, 440nm, 470nm. Continuous 440nm, 488nm, 514nm and 552nm
(Raman) 532nm, 785nm.

Objectives:

(SP8+Raman) 20x dry, 20x 0.75NA multi-immersion, 25x 0.95NA water dipping, 63x 1.2NA water, 63x 1.4NA oil.
(Raman only) 5x dry, 20x dry, 50x dry, 20x 0.75NA multi-immersion.

Coronavirus

 

SLCU Latest Updates

(for staff & students)

 

University of Cambridge Guidance 

 

We would like to thank NHS staff, key workers and volunteers who are working tirelessly throughout the ongoing coronavirus pandemic in the UK. Our thoughts are with those whose health is impacted here in the UK and around the world.

 

 

Supported by the Gatsby Charitable Foundation

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