skip to primary navigationskip to content
 

Leica-Renishaw SP8-FLIMan

A partnership between SLCU and three companies; Leica Microsystems, Leica Industrial and Renishaw, the FLIMan allows a diverse range of specialist techniques in addition to high-end confocal microscopy:

- Real time imaging of protein-protein interactions and biosensors using FLIM.

- Label-free organelle imaging using Raman, in addition to fluorescently labelled reporters.

- Direct imaging of molecular constituents of cells and tissues with applications to phytohormones.

Equipment

Leica SP8 confocal with dual channel FLIM, 2x HyD SMD detectors, 2x PMTs, 1x t-PMT, automatic stage for use in both confocal and Raman modes. Renishaw InVia Raman system with Rencam CCD and Andor EMCCD detectors. Conversion kit for liquid samples.

Software

Leica LAS X with FLIM module, Picoquant software for photon counting. WiRE software for Raman spectral acquisition and
mapping.
 

Lasers:

(Confocal) Pulsed 405nm, 440nm, 470nm. Continuous 440nm, 488nm, 514nm and 552nm
(Raman) 532nm, 785nm.

Objectives:

(SP8+Raman) 20x dry, 20x 0.75NA multi-immersion, 25x 0.95NA water dipping, 63x 1.2NA water, 63x 1.4NA oil.
(Raman only) 5x dry, 20x dry, 50x dry, 20x 0.75NA multi-immersion.

 

Supported by the Gatsby Foundation

RSS Feed Latest news

SLCU helps reveal another layer in the strigolactone signalling pathway

Nov 23, 2018

An interdisciplinary collaboration between structural biologists and plant scientists has revealed another layer in the signalling pathway of strigolactone – a plant hormone that plays a key role in shoot branching and other plant development processes.

Circadian clock imparts continuous control over the timing of cell division

Nov 15, 2018

SLCU researchers have demonstrated that the circadian clock is more complex than a simple ‘on-off switch’ when it comes to controlling cell division.

SLCU recognised for commitment to gender equality

Oct 25, 2018

Sainsbury Laboratory Cambridge University (SLCU) has been recognised for its ongoing commitment to gender equality by achieving a Silver Award under the Athena SWAN Charter.

View all news